Catalog Number - 68312-05P-2

Cogent RP C12™ is a unique reversed-phase HPLC stationary phase designed to provide balanced hydrophobic retention, rapid equilibration, and excellent reproducibility. Manufactured using a rigid adamantane-shaped carbon silane bonded to ultra-high purity silica and fully double end capped, this stationary phase offers selectivity that falls between traditional C8 and C18 phases. The distinctive ligand structure provides excellent performance for complex mixtures and applications requiring robust retention behavior across a wide range of mobile-phase conditions.

68312-05P-2 is an HPLC column packed with 3 µm RP C12™ particles featuring a 100 Å pore size in a 2.1 mm ID x 50 mm format. This short, narrow-bore configuration is optimized for rapid separations, reduced solvent consumption, and excellent compatibility with LC-MS instrumentation. The 3 µm particles provide greater efficiency and sharper peaks than comparable 5 µm columns, while the short 50 mm length minimizes run times for high-throughput workflows. Typical operating flow rates are approximately 0.2–0.3 mL/min. Because of the low flow rates required by 2.1 mm ID columns, standard HPLC systems must be capable of precise and reproducible low-flow operation.

Compatible with virtually all reversed-phase mobile phases, including 100% aqueous conditions, this column maintains consistent retention without the phase collapse often associated with some traditional reversed-phase columns. Retention is typically greater than C8 phases and lower than C18 phases for the same hydrophobic compounds. This format is especially popular for LC-MS applications, high-throughput screening (HTS), and rapid analytical methods.

Key Benefits

• RP C12™ stationary phase

• Fully double end capped

• Adamantane-shaped bonded ligand

• Ultra-high purity silica support

• Compatible with 100% aqueous mobile phases

• Rapid equilibration between runs

• 3 µm particle size

• Greater efficiency than 5 µm columns

• Excellent for LC-MS and HTS methods

• Retention characteristics between C8 and C18 phases

Typical Applications

• High-throughput screening (HTS)

• LC-MS methods

• Reversed-phase HPLC

• Complex mixture separations

• Pharmaceutical analysis

• Method development

• Quality control testing

• Hydrophobic compound analysis

• Gradient separations

• Research and development

Specifications

• Product Type: HPLC Column

• Stationary Phase: RP C12™

• Chromatography Mode: Reversed Phase

• Bonded Phase: Adamantane Carbon Silane

• Endcapping: Fully Double End Capped

• Particle Size: 3 µm

• Pore Size: 100 Å

• Column Dimensions: 2.1 mm ID x 50 mm

• Length: 50 mm

• Typical Flow Rate: 0.2–0.3 mL/min

• Brand: Cogent

Important Notes

• Compatible with 100% aqueous mobile phases

• Maintains consistent retention from run to run

• Rapid equilibration supports high-throughput workflows

• The 2.1 mm ID format is commonly used for LC-MS applications

• Standard HPLC systems must be capable of precise and reproducible operation at approximately 0.2–0.3 mL/min

• Detector flow cells should be optimized for low-volume, narrow-bore chromatography

• 3 µm particles provide higher efficiency and sharper peaks than comparable 5 µm columns

• Generates higher backpressure than larger particle columns

• The 50 mm length is ideal for rapid analyses and increased sample throughput

• Retention is generally greater than C8 phases and less than C18 phases